Article_5_2_3

                   ORTHODONTICS
                   SOFT-TISSUE FACIAL ASYMMETRY BEFORE AND AFTER ORTHOGNATHIC SURGERY:
Original Article
                   APPLICATION OF A NEW 3D PROTOCOL
                   Filippo Da Pozzo1,2a, Francesca M.E. Rusconi1b, Giada Anna Beltramini2c, Daniele M. Gibelli1d, Valentina Pucciarelli1e,
                   Aldo Bruno Giannì2,3f, Chiarella Sforza1g
                   1
                    LAFAS, Laboratory of Functional Anatomy of the Stomatognathic System, Department of Biomedical Sciences for Health, Università degli Studi di Milano,
                   Milan, Italy
                   2
                    Maxillofacial and Dental Unit, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico di Milano, Milan Italy
                   3
                    Department of Biomedical, Surgical and Dental Sciences, University of Milan, Milan Italy

                   a
                     MD
                   b
                     DDS
                   c
                     MD, PhD
                   d
                     MD, PhD, ORCID 0000-0002-9591-1047
                   e
                     MBiothec, PhD, ORCID 0000-0002-9165-133X
                   f
                    MD, PhD
                   g
                     MD, PhD, ORCID 0000-0001-6532-6464



                   ABSTRACT                                                 DOI: 10.25241/stomaeduj.2018.5(2).art.3
                   Introduction: Skeletal Class III patients often present a major facial asymmetry. In the
                   current investigation, a quantitative method to assess 3D facial asymmetry was applied                                       OPEN ACCESS This is an Open Access
                                                                                                                                                article under the CC BY-NC 4.0 license.
                   to an orthognathic surgery patient to quantify possible postsurgical modifications.                                           Peer-Reviewed Article
                   Methodology: Soft-tissue facial scans of a 20-year-old man with skeletal Class III,
                                                                                                                                           Citation: Da Pozzo F, Rusconi FME, Beltramini
                   candidate to orthognathic surgery, were collected in the pre-surgery stage and 6, 12, 24                                GA, Gibelli DM, Pucciarelli V, Giannì AB, Sforza C.
                   months post-surgery with a stereophotogrammetric system. Soft tissue asymmetry was                                      Soft-tissue facial asymmetry before and after
                                                                                                                                           orthognathic surgery: application of a new 3D
                   calculated in the facial thirds according to a published protocol (J Craniomaxillofac Surg                              protocol. Stoma Edu J. 2018;5(2):98-101.
                   2017;45(1):76-81), and the relevant time-related modifications described. The results                                   Academic Editor: Constantinus Politis, MD,
                   were also compared to normal values from a group of 23 control subjects (10 men, 13                                     DDS, MM, MHA, PhD, Professor & Chairperson,
                   women, mean age 26) by using z-scores.                                                                                  University of Leuven, Leuven, Belgium

                   Results: The longitudinal analysis of the soft-tissue facial asymmetry showed a marked                                  Received: January 25, 2018
                                                                                                                                           Revised: March 14, 2018
                   difference in the analysed time points: orthognathic surgery did reduce facial symmetry                                 Acccepted: June 06, 2018
                   in the present patient. The comparison between the patient and the control subjects                                     Published: June 07, 2018

                   by using z-scores highlighted a clear difference in all-time points: the patient with facial                            *Corresponding author: Professor Chiarella
                   dysmorphia had a higher degree of asymmetry than healthy subjects.                                                      Sforza, MD, PhD, Department of Biomedical Sci-
                                                                                                                                           ences for Health, University of Milan, Milan, Italy,
                   Conclusion: The measurements of soft-tissue facial asymmetry using 3D optical                                           via Mangiagalli 31, I-20133 Milano, Italy, Tel: +39
                   digitisers can provide clinically useful information. The graphical representation of                                   – 02 503 15385, Fax: +39 – 02 503 15387, e-mail:
                                                                                                                                           chiarella.sforza@unimi.it
                   results can help in the patient’s understanding of the treatment phases, thus increasing
                   compliance.                                                                                                             Copyright: © 2018 the Editorial Council for the
                                                                                                                                           Stomatology Edu Journal.
                   Keywords: face, soft tissues, orthognathic surgery, symmetry.

                   1. Introduction                                                               surgeons, the orthodontists and the patients, allowing
                   Facial dysmorphoses are not common in the western                             the best compliance especially for treatments that
                   world. According to US studies, dentoskeletal Class III                       require several interventions, and that need a middle
                   patients can be found in 0.6% of the general population,                      term follow up to show their final results [6,7].
                   a much smaller value than that reported for dentoskeletal                     Current technology offers a wide range of computerized
                   Class II patients, which is around 10% [1].                                   optical scanners that can be used to assess facial soft
                   Nonetheless, Class III patients often present more                            tissues not invasively, providing a 3D, quantitative
                   complex situations than Class II ones, with a much                            reproduction of the patients’characteristics [8].The digital
                   more altered facial function and aesthetics, and                              images can be evaluated longitudinally to appreciate the
                   the percentage of patients undergoing surgical                                effects of the various phases of the treatment [6] and can
                   interventions is approximately 6-7 times larger in Class III                  be compared to those obtained from normal subjects of
                   than in Class II subjects (about 33% vs. 5%) [1]. Apart from                  comparable age, sex and ethnicity.
                   the restoration of a sound functionality and a normal                         Indeed, the need for reference values is important,
                   occlusion, aesthetics and facial symmetry are among                           as a slight facial asymmetry is also present in healthy
                   the most important aspects in modern orthognathic                             subjects [9-11], and even the best surgical and
                   surgery [2]. The successful attainment of an aesthetically                    orthodontical approaches cannot restore a perfectly
                   acceptable result starts from correct skeletal and dental                     symmetrical appearance. In the present study, we
                   positions [3-5], but it needs a thorough evaluation of the                    evaluated the soft tissue facial asymmetry of a patient
                   facial soft tissues [1,2]. The external appearance of the                     undergoing a combined surgical-orthodontic treatment
                   face is also the key point for a fruitful dialogue among the                  to correct a Class III dento-skeletal malocclusion. 3D




   98                                                                           Stoma Edu J. 2018;5(2): 98-101                     http://www.stomaeduj.com
                                  SOFT-TISSUE FACIAL ASYMMETRY BEFORE AND AFTER ORTHOGNATHIC SURGERY:
                                                                      APPLICATION OF A NEW 3D PROTOCOL


morphometric measurements were performed on




                                                                                                                                              Original Article
digital stereophotogrammetric images, and facial soft
tissue asymmetry quantified. Data were obtained
longitudinally and compared to those obtained in
healthy, control subjects.


2. Materials and methods
The facial soft tissues of a 20-year-old man were
imaged by using the VECTRA M3 3D Imaging System
(Canfield Scientific, Fairfield, NJ, USA). The instrument is
a stereophotogrammetric unit made up of three pods.
In each pod, there are two cameras that photograph
the subject’s face from different points of view (Fig. 1).
A previous calibration permits to obtain the metrical
data of the facial surface (from ear to ear, from trichion       Figure 1. VECTRA M3 3D Imaging System (Canfield Scientific, Fairfield, NJ,
to neck) in a few milliseconds. Files can be exported           USA): the three pods that surround the subject can be seen.
and elaborated with proprietary and custom software.
In particular, the Mirror® Vectra Software (Canfield
Scientific, Fairfield, NJ, USA) was used in the present
investigation [4].
The patient had a diagnosis of skeletal Class III
dysmorphism and was a candidate for a surgical
intervention of bimaxillary osteotomy. The patient was
analysed in 4 different stages (preoperative, at the end of
orthodontic treatment, and postoperatively 6, 12 and 24
months after surgery). On each occasion, he was imaged
while seating with a natural relaxed expression (closed
lips, teeth in slight contact).
A group of 23 subjects were selected from the
Laboratory archive to form the control group (13 females
and 10 males, mean age 26, SD 6.8 years); they all had
a diagnosis of dentoskeletal Class I and no history of
traumas or alterations in the facial bones. Acquisitions
were obtained after the patients’ written informed
consent and did not involve any invasive, painful or
dangerous procedure. All procedures were performed as
previously described in the literature [12,13]. In brief, on
each facial image, a set of 50 anthropometric landmarks
were identified and digitized. Afterwards, following the
protocol validated by Codari et al. [3], the anterior part of
the face was delimitated by the following 10 landmarks:
trichion (tr); right and left frontotemporal (ft); right and
left zygion (zy); right and left tragion (t); right and left     Figure 2. Landmarks used for symmetry analysis. (Written consent for the
                                                                publication of this image was obtained).
gonion (go); gnathion (gn) (Fig. 2).
Each half-face was split into three thirds (upper, middle       The work described was carried out in accordance with
and lower part); the mid facial plane of maximum                The Code of Ethics of the World Medical Association
symmetry was automatically obtained, and the two                (Declaration of Helsinki). Informed consent was obtained
facial halves superimposed. The software automatically          from all patients, and their privacy rights observed. Ethi-
calculated the Root Mean Square (RMS) distance values           cal approval was given by University ethics review board.
among the two facial halves separately for each facial
third, thus providing a set of symmetry values. The
lower the value, the higher the symmetry. Ideally, very         3. Results
symmetrical areas should have an RMS value of 0.                Table 1 illustrates the time-related variations in facial
To assess the longitudinal modifications, the preopera-         asymmetry in the analysed patient. A trend of improve-
tive image was superimposed on those obtained at 6, 12          ment in his facial symmetry was observed during the
and 24 months after the intervention, and RMS values            follow-up examinations.
obtained. The software also produced colorimetric maps          With regards to the lower third of the face, the RMS value
which highlight the modifications in an intuitive way.          obtained from the overlap of the pre-surgical images
The same procedures were followed for the subjects of           of the two hemifaces was 1.2 mm; this value decreased
the control group, and descriptive statistics (mean, SD)        in the subsequent acquisitions made after 6, 12 and 24
were obtained for regional facial asymmetry and used to         months. A reduction in the RMS values was also found in
calculate z-scores (Patient value minus reference mean          the middle and upper facial thirds.
value divided by reference SD). The smaller the z-score,        When the facial asymmetry of the patient was compared
the similar the patient is to the control group.                to that of healthy, reference subjects using z-scores, a
IRB Approval                                                    trend of improvement was seen after surgery (Fig. 3). The



Stomatology Edu Journal                                                                                                                          99
                   SOFT-TISSUE FACIAL ASYMMETRY BEFORE AND AFTER ORTHOGNATHIC SURGERY:
                   APPLICATION OF A NEW 3D PROTOCOL

Original Article    Table 1. Time-related variations in facial asymmetry in the analysed
                   patient (mm). The larger the value, the more asymmetrical the patient.
                                                      RMS 6        RMS 12        RMS 24
                                      RMS PRE
                                                     months        months        months
                     Upper third
                                          0.887         0.753         0.831         0.876
                      (forehead)
                    Middle third
                                          1.300         0.640         0.691         0.813
                       (maxilla)
                     Lower third
                                          1.213         0.699         0.699         0.974
                     (mandible)




                    Figure 3. Z-scores of the analysed patient during the 24-months follow
                   up.

                   z-score value calculated in pre-surgical acquisitions was
                   1.28 SD for the lower third, 1.79 SD for the middle third                   Figure 4. Localised asymmetry in the upper, middle and lower parts of
                   and 0.38 SD for the upper third; 24 months after surgery                  the face in the analysed patient. The pre-surgical image is compared to that
                   the z-score was 0.52 SD for the lower third, 0.18 SD for                  obtained at the 24 months follow up..
                   the middle and 0.37 SD for the upper one.                                 more than 6 months [15].
                   To better assess the localised facial asymmetry, Fig. 4                   Generally, patients with skeletal dysmorphia are more
                   presents the asymmetry divided in the upper, middle                       asymmetrical than healthy subjects without malocclu-
                   and lower parts of the face in the analysed patient. The                  sions [9,16], as shown in the current analysis that allowed
                   pre-surgical image is compared to that obtained at the                    to localize those parts of the face showing a higher de-
                   24 months follow up. The software produces a colori-                      gree of left-right imbalance. The present method can,
                   metric map which highlights the variations in an intui-                   therefore, be coupled with the conventional analyses of
                   tive manner. The major differences are represented in                     facial esthetics [17], thus increasing the quantitative de-
                   red and in blue, respectively by default and in excess.                   scription of the patient, and helping in treatment plan-
                   The unvaried areas are coloured in green. As expected                     ning and monitoring of follow up.
                   the degree of asymmetry is reduced in every third of the
                   face and the best results are seen in the lower third.
                                                                                             5. Conclusion
                                                                                             The measurements of soft-tissue facial asymmetry using
                   4. Discussion                                                             3D optical digitisers can provide clinically useful infor-
                   In the current investigation, we performed a longitudi-                   mation. The graphical representation of results can help
                   nal evaluation of soft-tissue facial asymmetry in a patient               in the patient’s understanding of the treatment phases,
                   candidate for bimaxillary surgery for a skeletal Class                    thus increasing compliance.
                   III malocclusion. The method used a set of stereopho-
                   togrammetric facial scans, and it allowed a complete
                   picture of the modifications of the patients’ soft tissues:               Author Contributions
                   orthodontists and surgeons are thus provided with an                      FP: Conception and design of the study; Acquisition
                   extra tool to plan and monitor the clinical outcome. An                   of data; Analysis and interpretation of data collected;
                   additional advantage is the patient-friendly depiction                    Drafting of the article; Final approval. FMER: Acquisition
                   of the results which can enhance the patient’s compre-                    of data; Analysis and interpretation of data collected;
                   hension of the various treatment phases, with increased                   Drafting of the article; Final approval. GAB: Acquisition
                   compliance [6].                                                           of data; Analysis and interpretation of data collected;
                   The acquisitions were made with a middle term follow                      Drafting of the article; Final approval. DMG: Acquisition
                   up, starting in the preoperative (post orthodontic) phase,                of data; Analysis and interpretation of data collected;
                   and subsequently 6, 12 and 24 months after surgery. The                   Drafting of the article; Final approval. VP: Analysis and
                   24-months stage allowed stable facial images of the                       interpretation of data collected; Drafting of the article;
                   patients to be obtained, without the transient effects                    Final approval. ABG: Conception and design of the
                   of surgery on soft tissues edema and the post-surgical                    study; Analysis and interpretation of data collected;
                   orthodontic treatment [1,14]. Indeed, despite the vari-                   Critical revision of article; Final approval. CS: Conception
                   ety of studies on 3D facial asymmetry after bimaxillary                   and design of the study; Analysis and interpretation of
                   orthognathic surgery, few were longitudinal and lasted                    data collected; Critical revision of article; Final approval.



 100                                                                            Stoma Edu J. 2018;5(2): 98-101                  http://www.stomaeduj.com
                                         SOFT-TISSUE FACIAL ASYMMETRY BEFORE AND AFTER ORTHOGNATHIC SURGERY:
                                                                             APPLICATION OF A NEW 3D PROTOCOL

                                                                                  pediatric population. J Biol Regul Homeost Agents. 2017;31(2




                                                                                                                                                              Original Article
                                                                                  Suppl 1):131-138.
Acknowledgments                                                                   [PubMed] Google Scholar(2) Scopus(0)
The authors have no conflicts of interest.                                  8.    Sforza C, de Menezes M, Ferrario V. Soft- and hard-tissue facial
This research did not receive any specific grant from                             anthropometry in three dimensions: what's new. J Anthropol Sci.
                                                                                  2013;91:159-184. doi:10.4436/jass.91007.
funding agencies in the public, commercial, or not-for-                           [Full text links] [PubMed] Google Scholar(45) Scopus(27)
profit sectors. The authors declare that the research                       9.    Cheong YW, Lo LJ. Facial asymmetry: etiology, evaluation, and
was conducted in the absence of any commercial or                                 management. Chang Gung Med J. 2011;34(4):341-351.
                                                                                  [PubMed] Google Scholar(109) Scopus(50)
financial relationship that could be construed as a                         10.   Ferrario VF, Sforza C, Dellavia C, et al. A quantitative three-
potential conflict of interest.                                                   dimensional assessment of soft tissue facial asymmetry of cleft lip
                                                                                  and palate adult patients. J Craniofac Surg. 2003;14(5):739-746.
                                                                                  [Full text links] [PubMed] Google Scholar(49) Scopus(37)
                                                                            11.   Sforza C, Laino A, D'Alessio R, et al. Soft-tissue facial characteristics
References                                                                        of attractive Italian women as compared to normal women. Angle
1.   Proffit WR, White RP, Sarver DM. Contemporary treatment of                   Orthod. 2009;79(1):17-23. doi: 10.2319/122707-605.1.
     dentofacial deformity. 15th ed. St. Louis, Mo: Mosby. Chicago;               [Full text links] [PubMed] Google Scholar(49) Scopus(26)
     2003.                                                                  12.   Codari M, Pucciarelli V, Stangoni F, et al. Facial thirds-based
     Google Scholar(412)                                                          evaluation of facial asymmetry using stereophotogrammetric
2.   Cullati F, Mapelli A, Beltramini G, et al. Surface electromyography          devices: Application to facial palsy subjects. J Craniomaxillofac
     before and after orthognathic surgery and condylectomy                       Surg. 2017;45(1):76-81. doi: 10.1016/j.jcms.2016.11.003.
     in active laterognathia: a case report. Eur J Paediatr Dent.                 [Full text links] [Free full text] [PubMed] Google Scholar(11)
     2017;18(2):131-138. doi: 10.23804/ejpd.2017.18.02.08.                  13.   De Menezes M, Rosati R, Ferrario VF, et al. Accuracy and
     [Full text links] [PubMed] Google Scholar(0) Scopus(0)                       reproducibility of a 3-dimensional stereophotogrammetric
3.   Chew MT. Spectrum and management of dentofacial deformities                  imaging system. J Oral Maxillofac Surg.2010;68(9):2129-2135. doi:
     in a multiethnic Asian population. Angle Orthod. 2006;76(5):806-             10.1016/j.joms.2009.09.036.
     809. doi: 10.1043/0003-3219(2006)076[0806:SAMODD]2.0.CO;2                    [Full text links] [PubMed] Google Scholar(91) Scopus(58)
     [Full text links] [PubMed] Google Scholar(52) Scopus(24)               14.   Aydemir H, Efendiyeva R, Karasu H, Toygar-Memikoğlu U.
4.   Gibelli DM, Pucciarelli V, Pisoni L, et al. Quantification of dental         Evaluation of long- term soft tissue changes after bimaxillary
     movements in orthodontic follow-up: a novel approach based                   orthognathic surgery in Class III patients. Angle Orthod.
     on registration of 3D models of dental casts. Stoma Edu J.                   2015;85(4):631-637. doi: 10.2319/062214-449.1.
     2017;4(1):53-59. doi: 10.25241/stomaeduj.2017.4(1).art.5.                    [Full text links] [PubMed] Google Scholar(4) Scopus(3)
5.   Shaheen E, Politis C. The use of 3D virtual planning and               15.   Kobayashi T, Ueda K, Honma K, et al. Three-dimensional analysis
     3D printing for the treatment of facial asymmetry: a case                    of facial morphology before and after orthognathic surgery. J
     report. Stoma Edu J. 2016;3(3-4):156-161. doi: 10.25241/                     Craniomaxillofacial Surg. 1990;18(2):68-73.
     stomaeduj.2016.3(3-4).art.4.                                                 [Full text links] [PubMed] Google Scholar(51) Scopus(25)
     Google Scholar(1)                                                      16.   Chew MT. Spectrum and management of dentofacial deformities
6.   Pucciarelli V, Tarabbia F, Codari M, et al. Stereophotogrammetric            in a multiethnic Asian population. Angle Orthod. 2006;76(5):806-
     evaluation of labial symmetry after surgical treatment of a                  809. doi: 10.1043/0003-3219(2006)076[0806:SAMODD]2.0.CO;2.
     lymphatic malformation. J Craniofac Surg. 2017;28(4):e355-e358.              [Full text links] [PubMed] Google Scholar(52) Scopus(24)
     doi: 10.1097/SCS.0000000000003601.                                     17.   Rossetti A, De Menezes M, Rosati R, Ferrario VF, Sforza C. The role
     [Full text links] [PubMed] Google Scholar(1) Scopus(2)                       of the golden proportion in the evaluation of facial esthetics.
7.   Segna E, Pucciarelli V, Beltramini GA, et al. Parry Romberg                  Angle Orthod. 2013;83(5):801-808. doi: 10.2319/111812-883.1.
     Syndrome and linear facial scleroderma: management in                        [Full text links] [PubMed] Google Scholar(33) Scopus(13)



                                                                                                Filippo DA POZZO
                                                                                                         MD
                                    LAFAS, Laboratory of Functional Anatomy of the Stomatognathic System
                                          Department of Biomedical Sciences for Health, University of Milan
                                                                    via Mangiagalli 31, I-20133 Milano, Italy


CV
Filippo Da Pozzo, MD, graduated in 2017 with the highest grades in Medicine and Surgery at the University of Milan. Since
2015 he has been attending the university department of Oral and Maxillofacial surgery at the polyclinic hospital Fondazione
IRCCS Ca' Granda in Milan. He is currently a member of the research staff in the Laboratory of Functional Anatomy of the
Stomatognathic System - LAFAS, Department of Biomedical Sciences for Health at the University of Milan, Milan, Italy. His fields
of research are the morphological and metrical assessment of anatomical characteristics of craniofacial structures, including
their modifications with pathology and treatment.

Questions                                                                   3. In the current study we investigated
                                                                            qa.  Three-dimensional modifications in dental arch
1. Which instruments can be used to                                         diameters;
                                                                            qb.  The effect of operator experience in making
measure soft tissue facial asymmetry?                                       facial measurements;
qa. Bite wing radiographs;                                                  qc.  The use of Computerized Tomography to
qb. Lateral plane teleradiographs;                                          measure facial asymmetry;
qc. Stereophotogrammetric units;                                            qd. A new protocol to assess localized soft-tissue facial
qd. Conventional orthopantomographs.                                        asymmetry.
2. How was facial asymmetry                                                 4. In the current investigation, we found that:
measured?                                                                   qa.  Soft-tissue facial asymmetry increased after
qa.  Using Root Mean Square distances among                                 dental extraction;
images;                                                                     qb.  Skeletal facial asymmetry decreased after
qb.  Using surface electromyography;                                        implant placement;
qc.  Superimposing facial photos to Computerized                            qc.  Soft-tissue facial asymmetry decreased after
Tomography reconstructions;                                                 orthognathic surgery;
qd.  Measuring the distances between selected                               qd.  Dental arch asymmetry increased after functional
skeletal landmarks.                                                         orthodontic treatment.


Stomatology Edu Journal                                                                                                                                        101