articol-spaveras-2017

           CARIOLOGY
           CARIES DETECTION WITH LASER FLUORESCENCE DEVICES. LIMITATIONS OF THEIR USE
Practice
           Andreas Spaveras1a, Angeliki Tsakanikou2b, Frantzeska Karkazi3c, Maria Antoniadou1d*
           1
               Department of Operative Dentistry, Dental School, National and Kapodistrian University of Athens, Greece
           2
               Department of Operative Dentistry, Dental School, Semmelweis University, Budapest, Hungary
           3
               Department of Operative Dentistry, Dental School, Comenius University of Bratislava, Slovakia

           a
             DMD, Postgraduate Student
           b
             DMD
           c
            MD Dr
           d
             DDS, PhD, Assistant Professor


                                                                                                                                                                           Received: March 09, 2017
                                                                                                                                                                             Revised: March 24, 2017
                                                                                                                                                                            Accepted: April 02, 2017
                                                                                                                                                                            Published: April 03, 2017

           Academic Editor: Dana Cristina Bodnar, DDS, PhD, Professor, “Carol Davila” University of Medicine and Pharmacy Bucharest, Bucharest, Romania


           Cite this article:
           Spaveras A, Tsakanikou A, Karkazi F, Antoniadou M. Caries detection with laser fluorescence devices. Limitations of their use. Stoma Edu J. 2017;
           4(1):44-51.

           ABSTRACT                                                                                             DOI: 10.25241/stomaeduj.2017.4(1).art.4
           Background: Dental caries is one of the most prevalent human diseases worldwide. The modern
           concept of minimal invasive dentistry includes early detection of incipient caries lesions and its
           treatment. Several optical and digital detection methods are available.
           Objective: This literature review presents the utility and limitations of laser fluorescence caries
           detection devices DIAGNOdent (DD) and DIAGNOdent Pen (DDpen) (KaVo Dental GmbH, Biberach/
           Riβ, Germany) for carious lesions on the occlusal surfaces of the permanent dentition.
           Data sources: All available in vitro and in vivo studies from Google Scholar, PubMed and Scopus
           machines corresponding to caries, DIAGNOdent, DIAGNOdent Pen and laser fluorescence as key
           words, were reviewed.
           Data extraction: Certain limitations of the studies were the inadequate analysis of the experimental
           protocols, the widespread sample use of the third molar, mistakes in sample handling and the limited
           number of studies evaluating the detection capability of DD and DDPen for secondary caries.
           Data synthesis: DD and DDPen are useful devices for caries detection on the occlusal tooth surfaces.
           Their main advantages are the very high reproducibility of measurements (>0.90), the ease of
           handling and the quantification and monitoring capacity. Their main limitations are the relatively low
           specificity for enamel lesions, the necessity of unstained surfaces and absence of plaque and pastes
           during measurements and the absence of a universal, clinically functional calibration value (COV).
           Conclusion: Further studies are required for more reliable data analysis and clinical interpretation
           of the relevant results.
           Keywords: caries, DIAGNOdent, DIAGNOdent Pen, laser fluorescence, laser.

           1. Introduction                                                                                          so frequent as compared to caries in pits and
           Dental caries is one of the most widespread                                                              fissures of the posterior teeth. Most commonly,
           human diseases around the world and one of                                                               occlusal caries occur more often in premolars
           the most important problems in contemporary                                                              and first molars.3,4 The difficulty of prompt clinical
           dentistry. The prevalence of dental caries is higher                                                     diagnosis in occlusal areas is due to the anatomical
           in the elderly and people of lower socioeconomic                                                         features of these surfaces as well as the use of
           status. Nevertheless, it affects not only children                                                       topical fluoride products. Fluoride can prevent
           but also adults.1 A substantial decline of caries                                                        the collapse of the superficial enamel layer and
           prevalence has been documented during the last                                                           influence the remineralization process. Therefore,
           decades, especially in the western world, primarily                                                      large dentine lesions might be less visible even
           due to multiple fluoride products and the caries                                                         when they have progressed substantially. This
           prevention methods available.2                                                                           phenomenon reaches the percentage of 10-40%
           Nowadays, dental caries in smooth and inter-                                                             in molars and it is described as “hidden caries”.5
           proximal surfaces of permanent dentition is not                                                          The caries disease is an imbalance of the

           *Corresponding author:
           Dr Maria Antoniadou, DDS, PhD, Assistant Professor, Department of Operative Dentistry, Dental School, National and Kapodistrian University of Athens, Greece.
           Thivon 2 Str., PO Box 11527, Goudi, Athens, Greece, Tel/Fax: +306.944.342.546, e-mail: mantonia@dent.uoa.gr




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                CARIES DETECTION WITH LASER FLUORESCENCE DEVICES. LIMITATIONS OF THEIR USE



dynamic processes of demineralization and                 diagnosis in occlusal surfaces.9,10,11 Lussi et al.12, in




                                                                                                                      Practice
remineralization of the teeth and in its initial stages   an vitro study, showed that the sensitivity of visual
it can be halted. Enamel demineralization is a daily      observation as a diagnostic method becomes
process that does not necessarily lead to caries.         double in case of dentine caries (0.62) when
Early intervention can turn an active lesion into         compared to that of enamel (0.31). Gimenez et
an inactive one. If the degree of demineralization        al.13 in a systematic review report that publications
does not exceed a certain point, the process may          in respect to the accuracy of optical observation as
come to a standstill, even if the enamel surface has      a ‘diagnostic instrument’ do not exhibit sufficient
been minimally affected. The conversion of a lesion       qualitative methodology as far as the selection of
from active to inactive requires early diagnosis          the samples is concerned. They also underline that
and careful monitoring, in order to minimize the          visual observation constitutes a reliable a clinical
restorative intervention.6 From this point of view,       caries detection method. In another systematic
modern caries detection means should permit               review, Bader et al.14 mention that the optical
monitoring of the caries process before the early         observation displays high specificity, but low
lesion progresses to an extensive cavity.                 efficiency for sensitivity and repeatability.
The main objective of this paper is to give an            As compared to the above laboratory studies,
overview of the use and utility of the two available      clinical studies report higher specificity than
caries detection devices of the occlusal surfaces of      sensitivity in response to visual observation.15
the permanent dentition, DIAGNOdent (DD) and              The difficulty with the clinical studies is the lack of
DIAGNOdent Pen (DDPen) (KaVo Dental GmbH,                 proper identification of the healthy dental tissues
Biberach/Riβ, Germany), whose function is based           by the devices due to the frequent presence
on the fluorescence laser beam.                           of dental calculus, bacterial plaque, saliva and
                                                          food remnants. The use of established optical
2. The criteria for the evaluation of caries              calibration systems such as ICDASII or Ekstrand’s
diagnostic means                                          criteria seems to contribute to a more accurate
The criteria used for the diagnostic evaluation of        caries detection system, given the fact that they
caries are expressed through specific indicators          provide guidelines and a rational quantification of
which are defined in numerical scales and form            lesions.15 The combined use of visual observation
the diagnostic accuracy of a test. Specificity and        and tactile sensation with the use of metal probe
sensitivity are the two dimensions, widely used           does not appear to significantly improve the
for the description and quantification of several         diagnostic capability of direct visual observation.
diagnostic techniques. Specificity refers to the          Agnes et al. emphasize the possibility of damaging
correct identification of the healthy dental tissues,     the adjacent tooth with the sharp edge of the
while sensitivity refers to the correct identification    probe.16 On the other hand, the visual observation
of caries. The above indicators are expressed as          assisted by loops of 1,5X to 4,5X shows increased
values between 0 and 1 (100%). As these values            detection sensitivity.17
approach 1, the qualitative effect is higher and they     Bitewing radiographs are a useful means of
should be at least 0.75 for sensitivity and above 0.85    detecting interproximal caries; however, their
for specificity.7 Methods with low sensitivity can        advantage is quite limited to occlusal carious
lead to suboptimal treatment, whereas methods             lesions, due to the overlay phenomenon.18
with low specificity, to overtreatment. The caries        Furthermore, 40-60% of the tooth’s metal ions
detection methods with low sensitivity should be          have to be lost so as the lesion becomes visible
combined with techniques that are distinguished           radiographically. This is another reason why the
by high specificity and vice versa.                       radiographic imaging is not used clinically for the
The accuracy of a method in most studies occurs           detection of incipient caries lesions.19
from the sensitivity and specificity values and is        Therefore, the most important limitations of
often described by the area below the ROC curve           conventional diagnostic tools are their low
(Receiver Operating Characteristics). The accuracy        sensitivity, specificity and reproducibility, the
of the sum of the measurements obtained in                difficulty to determine the activity of the lesion
a procedure is called reliability or otherwise            and the inability to monitor its progression. For
repeatability and has a key role in the effectiveness     all these reasons, either more accurate detection
of the procedure.                                         methods or a combination of the above methods
2.1. Conventional caries diagnostic means                 should be used. In this respect, the dental
In everyday clinical practice, direct visual              technology has invested in the development of
observation is the most established method of             numerous caries detection devices. Some of these
tooth decay detection as it is easy and inexpensive.      diagnostic tools are based on infrared radiation,
This method is mainly based on the subjective             impedance spectroscopy, digital imaging as in
interpretation during visual examination and is           the DIFOTI system (Electro-Optical Sciences N.Y.),
often combined with radiographs and tactile               photo thermal radiometry as in the Canary System
examination with a metal probe.8                          (Quantum Dental Technologies, Canada) as well
Many studies indicate that visual observation is          as on visible spectrum fluorescence and laser
characterized by lower sensitivity in relation to         fluorescence.
specificity with the latter exceeding 0.85 for caries



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           CARIES DETECTION WITH LASER FLUORESCENCE DEVICES. LIMITATIONS OF THEIR USE



           2.2. Fluorescence of sound and carious tooth                with this technique.
Practice   substance
           Fluorescence is the property of a medium                    3. DIAGNOdent Device and DIAGNOdent Pen
           to absorb low wavelength radiation such as                  Device
           ultraviolet (1-400nm) emitting longer wavelengths           3.1. DIAGNOdent Device
           of visible light (430-450nm). Teeth have the                The light source of DD is a diode laser with a
           ability to emit fluoresce. This phenomenon can              wavelength of 655nm and a maximum power of
           be observed when the incident radiation is                  1mW. The red laser beam is transferred through
           shown in the ultraviolet spectrum, as in the cases          a descending optical fiber to its edge, made of
           of exposure to black light illumination or when             sapphire. Two different tip designs are available.
           the person is found at high altitude. The primary           The wedge-shaped which is used for occlusal
           fluorescence of teeth, otherwise known as auto-             surfaces and the straight one designed for smooth
           fluorescence, arises from the internal biological           dental surfaces. The excitation optical fiber, i.e.
           structures of the cells, with responsible elements          one that carries the light beam on the tissues,
           being several enzymes, vitamins, uranium glass              is surrounded by nine concentric optical fibers
           and endogenous fluorophores, present in dentin              of smaller diameter that collect the fluorescent
           and enamel.20 Dentin emits more autofluo-                   radiation together with the surrounding light
           rescence than enamel, with the emission peak                from the dental surfaces. All optical fibers have
           being at 450nm. Although the exact chemical                 a diameter of 40 microns and they are carved at
           mechanism of tooth auto-fluorescence has not yet            their end to receive or transmit the light radiation
           been ascertained, the greater amount of organic             in similar manner.25 A specially designed filter pre-
           components of dentine seems to be the reason for            vents the diffusion of ambient light (λ<655nm)
           its higher fluoresce values.21 It has been found that       and thus only the fluorescent light is collected and
           decayed tissues emits more fluoresce than healthy           converted into an electrical signal. Then, the signal
           ones upon stimulation by red laser or infrared              is displayed on two LED screens and expressed as
           irradiation. This seems to be the result of both            a integer number between 0 and 99. One screen
           demineralization processes and the presence of              displays the current measured value while the
           bacteria byproducts in the decayed tissue.22                other records the maximum value of detection.22
           2.3. Caries detection methods based on visible              3.1.1. Correlation detection values of DD
           spectrum fluorescence                                       Most clinical studies currently use the suggested
           Quantitative fluorescence (Quantitative Light –             measurements [Cut-Off Values (COV)] of the DD
           induced Fluorescence, QLF) is a method used                 as they appeared in the clinical study of Lussi et
           to detect the demineralization of enamel in the             al.12 In this study, seven examiners evaluated 332
           early stages. The technique relies on the ability of        occlusal surfaces of 240 patients. After histological
           enamel to emit strong auto-fluorescence under               examination, they found that the values between
           certain circumstances. Hypomineralised enamel               0-13 correspond to healthy dental tissues; values
           shows a decrease demission of fluorescence                  between 14-20 correspond to enamel caries
           spectrum as compared to that of healthy enamel.             and values between 21-29 to dentin caries.12 In
           With the use of the QLF method, demineralized               the same study, the restorative intervention is
           areas can be detected before they become                    suggested for values between 20-29. However,
           clinically visible, since the sensitivity of the specific   Tranaeus et al.26 suggested lower intervention
           technique is particularly high. Limitations of this         values.20-25 Anttonen et al.27 suggested intervention
           technique were found in the detection of dentine            values greater than 30, emphasizing that for values
           caries also in the deep enamel lesions (400μm),             greater than 40, the probability of overtreatment
           where the results were not so accurate.23                   is greatly reduced. Heinrich-Weltzien et al.28,
           2.4. Caries detection devices based on laser                compared the validity of various proposed COV,
           fluorescence                                                concluding that the superficial lesions in dentin
           The difference in fluorescence between sound                (D3) with rates between 17-21 showed the lowest
           and carious tooth structures was the fundamental            discrepancy (0,48 to 0,51). For deeper dentin
           concept behind the development of devices                   lesions (D4), the manufacturer’s suggested values
           capable of quantifying the decayed tissue                   (>34) had the best performance (0.51). Therefore,
           fluorescence. Methods based on fluorescence                 the proposed correlations of COV for DD vary
           are divided into those that use visible spectrum            considerably between studies and have changed
           stimulating rays such as the QLF and those based            several times even by the manufacturer. As a
           on laser ray fluorescence such as the DIAGNOdent            general observation, it is worth mentioning, that
           and the DIAGNOdent Pen (KaVo Dental GmbH,                   laboratory studies use lower COV for dentin caries
           Birebach/Riβ, Germany).23 Sundström et al.24 in a           in relation to clinical studies.
           pioneering study, stimulated carious and sound              3.1.2. Effect of exogenous factors on DD
           tooth structures by laser beams of different                measurements
           wavelengths (337nm, 488nm, 515nm, 633nm), and               Exogenous factors that could possibly influenceDD
           calculated the emitted fluorescence. The 488nm              values are various toothpastes and polishing
           wavelength was selected as the most appropriate             pastes. In an in vitro study, the potential effect of
           wavelength for the detection of incipient caries            ten different polishing pastes and four toothpastes



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                CARIES DETECTION WITH LASER FLUORESCENCE DEVICES. LIMITATIONS OF THEIR USE



on DD measurements was examined, after their              repeatability of the measurements for the DD




                                                                                                                   Practice
application to occlusal surfaces of molars and            between examiners was very high (0.957). Enamel
premolars. While toothpaste did not affect at all         (D1) and middle dentin (D3) have a specificity of
the DD values, seven of the polishing pastes have         D1:0.54, and D3:0.91 respectively, whereas the
an effect on the measurements with pumice being           sensitivity was D1: 0.91 and D3: 0.70. Therefore,
the leading one. It seems that the intense auto-          the ICDASII values were higher than those of DD.
fluorescence of certain polishing pastes may alter        The researchers conclude that combining ICDASII
the DD measurements, since their components               and DD investigating methods provide better
cannot be completely removed from the pits and            diagnostic results.
fissures of the occlusal surfaces of posterior teeth      The first combined in vivo/in vitro study for the DD
even after brushing and rinsing.29 Also, Lussi et al.30   device was conducted by Reis et al.35 who studied
in another in vitro study examined the influence          the caries detection of 57 third molars, both
of various toothpastes and prophylaxis paste              by direct visual observation and DD. The direct
remnants, as well as, powder remnants influencing         visual observation showed almost double in vivo
DD readings. The results of this experiment showed,       and in vitro repeatability, both between different
that only one toothpaste (Nupro mint/cherry               examiners (IR) (0.559) and between measurements
medium, Dentsply De Trey, USA) and one polishing          of the same examiner (IA) (0.559) compared to
paste (Clinic, 3M, Bioggio, Switzerland) had a            that of DD. This study shows higher sensitivity of
statistically significant effect on the measurements      DD measurements than the visual method, which
(p<0.01), after rinsing for 3-6 seconds. These            is not an usual finding in laboratory studies. The
formulations contain sticky elements, which in            presence of pigments in pits and fissures of the
combination with the high porosity of the decayed         occlusal surfaces may explain the above finding.
tissue, are not sufficiently removed and thereby          The authors suggest using 19-20 COV for the
increasing the DD measurements. If the teeth are          differentiation of healthy versus carious dentin.
not intensely rinsed with water-air combination for       They also proposed that the visual observation
at least ten seconds, an incorrect assessment may         using ICDASII is quite a reliable caries detection
occur. This is more significant for the long term         system.5
monitoring of lesions, rather than the detection of       Also in the study of De Paula et al.,35 visual
lesion per se.30                                          observation gave higher precision values than
3.1.3. Effect of sample storage means in the DD           the DD. These findings are in agreement with the
measurements                                              results by Rodrigues et al. and Agnes et al.36,16 The
The different storage means of the samples used           combination of detection techniques e.g. visual
in laboratory studies, such as chloramine solutions,      observation, radiography and DD seems though
formalin and thymol affect the final measurements         to result to more accurate diagnosis of caries as
of DD.31 Kaul et al.32 used 90 extracted molars in        mentioned also elsewhere.37 But it should be noted
groups of ten and stored the eight groups in eight        that the actual clinical experience of the operator
different solutions
              o
                       and one of them in a frozen        can affect the objectivity of the detection, either by
state of -20 C for one year. It was shown that the        visual observation or by using devices such as DD.
most reliable method for teeth storing was the            Specifically, in a laboratory study, 3 undergraduate
frozen state. According to this statement it has to       dental students, 3 general dentists and 3
be noted that only a few in vitro studies so far have     academics were asked to evaluate 25 molars by
used samples that were stored in a frozen state, a        visual observation and by using DD.
fact that should have an impact upon the clinical         The results showed a substantial variation. The
interpretation of the results.                            sensitivity of the measurements ranged from 0.188
3.1.4. Accuracy and repeatability of DD                   to 0.769 and the specificity from 0.714 to 0.969.
The characteristics of accuracy and repeatability of      The group of the academics recorded the highest
the measurements of the DD and DDPen devices              sensitivity of DD (0.667), while the group of the
are well documented. Chu et al.33 mention that            general dentists the highest specificity (0.942).
different COV values show different results. In an in     A substantial variation of measurements occurred
vivo study using COV by Lussi et al.,12 the sensitivity   in respect of sensitivity (0.755-0.953) and
(0.95) and specificity (0.11) differ considerably,        specificity (0.755-0.953) of the visual observation,
while for COV=40, the sensitivity (0.70) and              with the students reaching the greatest sensitivity
specificity (0.84) differ less. The authors propose       (0.80).38 Ideally, a detection technique for the
the combination of visual observation with the use        occlusal surfaces must have a very high sensitivity
of DD for caries detection, as it offers better results   for D3 and D4 and moderate high specificity for
in terms of specificity and quite good results in         detecting enamel caries.
respect to sensitivity.                                   The DD shows higher specificity for lesions at the
Jablonski-Momeni et al.,5 in an in vitro study,           level of dentin and lower for enamel lesions, since
examined 181 points of 100 posterior teeth                it is unable to identify the healthy tissues from the
comparing the DD detection capability with that           carious ones extending to the half of the enamel.
of direct visual observation during ICDASII. The



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           CARIES DETECTION WITH LASER FLUORESCENCE DEVICES. LIMITATIONS OF THEIR USE



           The sensitivity of DD can be increased for more         3.2.1. Accuracy and repeatability of DDPen
Practice   deep caries, with values of 0.66 in D2 and 1 in D3.     Lussi et al.40 compared in vitro the accuracy of
           Sensitivity for the D1 level was reported at 0.74.39    caries detection by DD and DDPen. In their study,    o
           3.2. DIAGNOdent Pen Device                              119 third molars, kept in frozen state at -20 C,
           The inability of DD to detect approximal caries was     were examined. DDpen showed higher specificity
           the primary cause of creating the DDPen (Fig.1).        (0.71 to 0.91) compared to the DD (0.69-0.79), but
                                                                   relatively lower sensitivity (0.78 to 0.91) against the
                                                                   latter (0.81 to 0.96).
                                                                   The main limitation of the study is that only third
                                                                   of the molars were used, whose occlusal surface
                                                                   varies considerably in different individuals as
                                                                   compared to other posterior teeth. Kuhnisch et
                                                                   al.41 found that the reproducibility of DD of the
                                                                   same examiner (0.89) was similar to that of DDPen
                                                                   (0.88), while between different examiners reliability
                                                                   (0.86) was noted.
                                                                   Sinanoglou et al.42 evaluated in vivo the occlusal
                                                                   surfaces of 217 permanent molars and premolars,
            Figure 1. The handpiece of the DDPen.
                                                                   comparing the visual observation (ICDASII), DDPen
                                                                   and bitewing radiography.
           The DDPen follows the basic principles of the DD        One week after the first measurements, the patients
           model. The main difference is the design of its tip     were invited for re-examination and 82 teeth were
           which can be rotated to the longitudinal axis and       reassessed with the above-mentioned techniques.
           thus permitting the detection of approximal caries.     Only the teeth with dentine caries were examined
           Also, DDPen uses the same optical sapphire fiber        (64 of 227) and the clinical depth of the lesion was
           for the distribution of radiation and the detection     measured.
           of tooth fluorescence without the interference of       The reliability of DDPen was moderate to good,
           other optical fibers (Fig. 2).                          with AUC 0.55-0.64, but noticeably inferior in
                                                                   contrast to that of visual observation (AUC 0.71-
                                                                   0.76) that reached higher specificity values than
                                                                   sensitivity. At this point, it should be mentioned that
                                                                   the results of the evaluation for visual observation
                                                                   could have been affected by the subjective skills
                                                                   and the level of the examiner’s acquaintance with
                                                                   the device.15
                                                                   Moreover, the device detection capability was
                                                                   better for dentin caries (D3), a finding supported
                                                                   by many other studies.14,15,43 The reproducibility for
                                                                   DDPen between different examiners (0.61, 0.65)
           Figure 2. The DDPen tip over an occlusal surface of a
                                                                   and the same one (0.59. 0.65) was relatively low
           molar.
                                                                   (16.42).
           Two different tips are available; a cylindrical one     It is worth noting that in the study by Seremidi
           (CYL) with a diameter of 1.1 mm and a conical one       et al.17 the teeth were stored in tap water for a
           (CON) with a diameter of 0.7mm. Although, the           long time, which is likely to have an impact on the
           diameter of the CON is about 0.3mm thinner than         fluorescence levels of the teeth.
           that of DD and thus it would be expected to show        The study by Achilleos et al.43 revealed low
           better accuracy on pits and fissures, it seems that     sensitivity values (0.66-0.75) for DDPen, which
           there is no significant difference between them40       may be attributed to the fact that the study was
           (Fig. 3).                                               focused on the D1 level, where the performance
                                                                   of this device is reduced compared to the D3
                                                                   level. Additionally, the relatively small number of
                                                                   samples38 and the only one week period among
                                                                   the two measurements were reported as limitations
                                                                   of this study.
                                                                   Mortensen et al.,44 focusing on the level of D3,
                                                                   showed high repeatability for DDPen between
                                                                   different examiners (0.98). For COV=40, there
                                                                   was a very high specificity (0.97) but very low
                                                                   sensitivity (0.07). The authors support the idea that
                                                                   if the manufacturer’s COV are applied in clinical
                                                                   practice, there will be a significant reduction of
                                                                   overtreatment, but also the detection of caries in
           Figure 3. The different tips of the DDPen.              D3 will be very low.



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                   CARIES DETECTION WITH LASER FLUORESCENCE DEVICES. LIMITATIONS OF THEIR USE



4. Secondary Caries detection with DD and                        radiographs. The statistically significant difference




                                                                                                                                   Practice
DDPen devices                                                    between the minerals of dentin presented in all
There are not many studies evaluating the                        evaluation periods (p>0.001) shows the highest
detection capability of DD and DDpen for                         value before the removal of caries (0.74) and the
secondary caries, reported exclusively on the                    lowest value after 12 months (0.04). This study
occlusal surfaces of permanent dentition. In a                   evaluated the DD measurements only through
2014 study, four examiners with different clinical               the demineralization rate of tooth without any
and dental experience reviewed 60 posterior                      disclosing measured values of DD or the results of
teeth restored with composite resin, by visual                   sensitivity, specificity and reproducibility.48
observation (Ekstrand criteria) and DDPen device.                As a result, although DD and DDPen show accurate
The reproducibility among the different examiners                measurements with high repeatability for the
was very high (0.954). The researchers concluded                 detection of secondary caries, most authors are
that DDPen is a reliable method for secondary                    reluctant to their use compared to primary means
caries detection and should be combined with                     such as the visual and radiographic examination
the visual observation for the correct diagnosis of              proposing their combined use.
secondary caries.45
Kositbowornchai et al.46 investigated the detection              5. Conclusions
capability of DD, under occlusal composite                       DD and DDPen are useful methods for occlusal
restorations, rather than tooth-resin interface.                 caries detection. Their main advantages are the
From the 100 teeth examined, only half were                      high reproducibility of measurements (>0.90), the
decayed and part of the caries was left on the                   ease of handling, the quantification of the carious
pulpal wall. All the teeth were restored with                    lesions and the monitoring ability. However,
composite resin (Z100 TM, 3 M, St. Paul, MN,                     they present significant limitations, such as the
USA) and the steps of etching and bonding                        relatively low specificity for enamel lesions, the
were omitted. The repeatability values between                   necessity of absence of stains, plaque and pastes
different examiners (from 0.60 to 0.77) were lower               during measurements and the absence of a single,
than that of Hamishaki et al.,45 while for the DD                clinically functional calibration value (COV). These
showed moderate sensitivity (0.74) and specificity               limitations support the view that these means are
(0.84). AUC value of the DD was moderate to good                 to be used as auxiliary in detecting or monitoring
(0.79) and higher than that of digital radiography               caries lesions of questionable activity. Ideally,
(0.65). Also there was no statistically significant              all optical and digital caries detection methods
difference in detection (p>0.05) between the two                 should have sensitivity, specificity, accuracy,
                                                                 repeatability, easiness in handling and access to all
means. So it was suggested that the amount of
                                                                 tooth surfaces. Nowadays, under the scope of the
fluorescence of composite resins does not affect
                                                                 minimally invasive dentistry, it seems necessary
the measurements of DD. However, the device
                                                                 for professionals to know and use both traditional
is only recommended as an auxiliary means of
                                                                 and newer methods for incipient caries’ detection
caries detection. These results are similar to an
                                                                 in order to avoid overtreatment. As in vivo and in
in vitro study which examined the diagnostic
                                                                 vitro data are based on methodological limitations,
capability of DD in 66 teeth with secondary caries,              further studies should be conducted estimating
of which 48 were restored with amalgam and 18                    the previous limitations and proceed with a more
with composite resin, where the sensibility was                  accurate evaluation of the specific devices.
0.77 and the specificity 0.81.47 In another in vivo
study, 30 molars were examined for the possible
development of secondary caries, 12 months after                 Acknowledgments
the restoration with glass-ionomer cement and                    The authors declare no conflict of interest related
amalgam. The diagnostic methods were the DD                      to this study. There are no conflicts of interest and
and the radiographic control by using bitewing                   no financial interests to be disclosed.

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                                                                                    Andreas SPAVERAS
                                                         Dentist, DMD, Postgraduate Student
                                                          Department of Operative Dentistry
                Dental School, National and Kapodistrian University of Athens, Athens, Greece




CV
Dr Andreas Spaveras received his degree in dentistry (DMD) in 2013 from the Semmelweis University of
Budapest, Hungary. Currently, he is a second year postgraduate student (MSc) at the Operative Department
of the National and Kapodistrian University of Athens, Greece. He is a member of numerous dental study
clubs such as the International Team for Implantology - ITI and has published several scientific articles. He is
an enthusiast of esthetic dentistry and photography.


Questions
Hidden caries are predominantely found in:
q    a.    Incisors;
q    b.    Canines;
q    c.    Premolars;
q    d.    Molars.
The caries diagnosis in everyday clinical practice is based on:
q    a.    Visual inspection;
q    b.    Laser fluorescence;
q    c.    Visible spectrum fluorescence;
q    d.    Infrared radiation.
The most appropriate wavelength for the detection of incipient caries by laser fluorescence is:
q    a.    337 nm;
q    b.    488 nm;
q    c.    515 nm;
q    d.    633 nm.
Which are the most appropriate Cut-Off Values of the DIAGNOdent for dentin caries:
q    a.    0-13;
q    b.    14-20;
q    c.    21-29;
q    d.    20-29.




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